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ORIGINAL ARTICLE
Year : 2018  |  Volume : 15  |  Issue : 4  |  Page : 286-290

Prevalence of hypothyroidism in type2 diabetic female Kurdish subjects


Department of Internal Medicine, College of Medicine, Hawler Medical University, Erbil, Iraq

Date of Web Publication20-Dec-2018

Correspondence Address:
Sarheed Jabar Muhammed
Department of Internal Medicine, College of Medicine, Hawler Medical University, Erbil
Iraq
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/MJBL.MJBL_99_18

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  Abstract 


Background: The incidence of thyroid disorders is more prevalent in type1 diabetes compared to type2 diabetes, due to associated autoimmune disorders. Physiological and biochemical interconnection between type1 diabetes mellitus and autoimmune thyroid disease is now stimulating subject of study. Objectives: The objective was to assess the prevalence of thyroid dysfunction among type2 diabetic Kurdish females and to explore the correlation between metabolic syndrome components and autoimmune thyroid abnormality. Materials and Methods: The study included 60 type2 diabetic Kurdish females and 30 sex-and age-matched controls. All patients in the study were exposed to anthropometric characteristics, including HbA1c, lipid profile, serum uric acid, thyroid-stimulating hormone(TSH), free triiodothyronine, free thyroxine, anti-thyroid peroxidase(TPO), and anti-thyroglobulin(anti-Tg). Results: Primary hypothyroidism was found in 24(40%) diabetic patients(5.67±3.35 μIU/mL) versus three(10%) controls (1.76±1.19 μIU/mL) (P<0.001). Anti-TPO was found in 66.7%(367.21±234.53) of diabetic patients versus 10%(31.78±32.14) of controls(P > 0.001). Anti-Tg was found in 60%(499.98±358.14) of diabetic patients versus 0(53.27±36.23) controls(P > 0.001). Asignificant positive relationship was estimated between both TPO antibodies and Tg antibodies and TSH(P=0.05 and P =0.001, respectively) in diabetic patients. Conclusion: Autoimmune thyroid disorder is more prevalent in Kurdish women with type2 diabetes than nondiabetic women, and thus points to a role of autoimmunity in the pathogenesis of type2 diabetes.

Keywords: Antithyroglobulin, anti-thyroid peroxidase, autoimmune thyroid dysfunction, stimulating hormone, type 1 diabetes mellitus


How to cite this article:
Muhammed SJ, Albustani DH. Prevalence of hypothyroidism in type2 diabetic female Kurdish subjects. Med J Babylon 2018;15:286-90

How to cite this URL:
Muhammed SJ, Albustani DH. Prevalence of hypothyroidism in type2 diabetic female Kurdish subjects. Med J Babylon [serial online] 2018 [cited 2019 Mar 23];15:286-90. Available from: http://www.medjbabylon.org/text.asp?2018/15/4/286/248057




  Introduction Top


Diabetes mellitus(DM) and thyroid diseases are the two of the most prevalent endocrine diseases encountered in clinical practice.[1] The WHO evaluation of diabetes incidence for all age groups worldwide was 2.8% in 2000 and 4.4% in 2030. The total number of people with diabetes is outlined to increase from 171 million in 2000 to 366 million in 2030.[2] Diabetes and thyroid disorders have been shown to commonly impact each other and the literature widely documented the association between these two conditions.[3]

Thyroid disorders are also very prevalent in the general population, and it is a common condition to affect the endocrine system. As a result, it is common for a patient to have both thyroid diseases and diabetes at the same time. The first study pointing out the interconnection between diabetes and thyroid dysfunction was published in 1979.[4] The prevalence of thyroid dysfunction among diabetes patients has been shown to be varying from 2.2% to 17%.[5]

Thyroid dysfunction includes hypothyroidism(Hashimoto's thyroiditis), hyperthyroidism(Grave's disease), and diffuse nodular goiter. Hypothyroidism and hyperthyroidism are induced by an autoimmune process, and they are involved in the spectrum of autoimmune thyroid disease. Autoimmune thyroid disease is common. The National Health and Nutrition Examination Survey study showed a high prevalence of hypothyroidism, and it is estimated to be 3.7%.[6] The prevalence of Graves' disease was reported to be 100–200cases per 100,000 populations per year. Previous papers have documented annual worldwide incidence of Hashimoto's thyroiditis be 0.30–1.5cases per 1000 persons.[7] It has been reported with advancing age, the frequency of thyroid dysfunction will be increased, and higher incidence of thyroid disease has been reported in female compared to male and in diabetic patients compared to nondiabetics.[4]

Perros et al. published an overall incidence of 13.4% of thyroid diseases in diabetics, with the highest rate in type1 DM (T1DM) females(31.4%) and lowest rate in T2DM males(6.9%). Thyroid disorders were pointed out to be more prevalent in individuals with T1DM compared to those with T2DM, due to the associated autoimmunity. Physiological and biochemical interconnections between T2DM and autoimmune thyroid disease are now an interesting field of research.[5]

There is no data on autoimmune thyroid diseases in T2D in Kurdish individuals; therefore, this study was performed to find out about autoimmune disease in T2D females.


  Materials and Methods Top


A descriptive cross-sectional case–control study was performed at the Department of Internal Medicine, Layla Qasim diabetic center and outpatient Department of Endocrinology, Hawler Teaching Hospital, Erbil, Iraq. The study was approved by Ethical Review Committee of Diabetic Association of Hawler Medical University. It included 60 T2DM Kurdish females living in iodine-sufficient area without a previously known thyroid disease and 30 sex-and age-matched nondiabetic healthy controls(age range 30–58years).

The patients were involved according to inclusion and exclusion criteria:

Inclusion and exclusion criteria

Inclusion criteria included female patients(Known case of T2DM), and the duration of DM≥5years. Exclusion criteria included the following:(1) patients taking drugs that influence the thyroid function,(2) postpartum women,(3) pregnancy, (4) known history of thyroid disease, and(5) unstable cardiac disease, renal impairment, liver cirrhosis, and malignancy.

An informed verbal consent was taken from each and every patient. All study participants were submitted to proper history taking, including duration of DM, current treatment, other comorbidities, and family history of DM and thyroid dysfunction.

Physical examination, including vital signs and general, head, neck, thyroid, extremities, chest, heart, abdominal, and neurological examination, was made. Anthropometric characteristics, including body mass index(calculated as weight in kilograms/height in square meters) and waist circumference, were made. The waist was measured at the midway point between the lowest rib plane and the iliac crest. Amedical history including duration of diabetes, age, sex, present diabetes conditions, and medication records was obtained. Height and weight were measured, and body mass index was calculated.

Laboratory investigations

With all aseptic precautions, 5ml venous blood was drawn from the anterior cubital vein in a disposal syringe and delivered in ethylenediaminetetraacetic acid tubes and mixed well.

The following laboratory investigations were performed: serum lipid profile(cholesterol[high-and low-density lipoprotein] and triglycerides) and serum uric acid using enzymatic assay(Boehringer, Mannheim, Germany); HbA1c using a kit supplied by Crystal Chem, Downers Grove, IL, USA); thyroid-hormone profile, including thyroid-stimulating hormone(TSH), free triiodothyronine(FT3), and free thyroxine(FT4), measured by enzyme-linked immunosorbent assay(DRG International, Springfield, NJ, USA);[8] antithyroid antibodies, including anti-thyroid peroxidase(TPO) and antithyroglobulin(anti-Tg), measured by enzyme-linked immunosorbent assay(Orgentec Diagnostika GmbH, Mainz, Germany).

Statistical analysis

Statistical analysis was performed using SPSS(IBM, Armonk, NY, USA) version22 and Microsoft Excel 2007. Results are expressed as means and standard deviation for quantitative data and as frequency(count) and relative frequency(percentage) for categorical data. Comparisons were done using unpaired t-tests. For comparison of categorical data, Chi-square tests were performed. Correlation was assessed by Pearson' correlation coefficient. P < 0.05 was considered statistically significant.


  Results Top


[Table1] shows comparability between DM patients(60) and controls(30) on clinical and anthropometric measures. There were statistically significant differences between the two groups in weight, body mass index, waist circumference, and systolic blood pressure.
Table 1: Comparison between patients and controls on clinical and anthropometric parameters

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[Table2] shows comparability between 60 diabetic patients and 30 controls on all laboratory data. There were highly statistically significant differences between the two groups in HbA1c, cholesterol(high-and low-density lipoprotein), uric acid, FT3, FT4, TSH, anti-TPO, and anti-Tg.
Table 2: Comparison between patients and controls on laboratory data

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[Table3] shows that among euthyroid diabetic patients(34), 9(25.0%) had both antibodies negative, 7(19.44%) had only anti-TPO positive, 6(16.66%) had only anti-Tg positive, and 14(38.88%) had both antibodies positive. Among controls that showed hypothyroidism, 3(100%) had both antibodies negative. Among those who had no hypothyroidism(24), 3(11.11%) had positive anti-TPO.
Table 3: Thyroid antibodies in patients and controls

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Among diabetic patients, who showed hypothyroidism(24), 3(12.5%) had negative antibodies, 5(20.8%) had only anti-TPO positive, 2(8.3%) had only anti-Tg positive, and 14(58.33%) had both antibodies positive. Among hypothyroid patients, a total of 24, 21(87.5%) patients showed overt hypothyroidism, while only 3(12%) showed subclinical hypothyroidism.

Correlations between both thyroid antibodies and all clinical and biochemical parameters in diabetic patients were significantly positive between TSH and anti-Tg(P=0.001), as shown in [Figure1], and TSH and anti-TPO(P=0.05), as shown in [Figure2].
Figure1: Correlation between antithyroglobulin and thyroid-stimulating hormone

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Figure2: Correlation between anti-thyroid peroxidase and thyroid-stimulating hormone

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  Discussion Top


The prevalence of autoimmune thyroid disease among T1DM is high.[9] However, thyroid dysfunction in T2DM is a less investigated field. Since normal thyroid function is mandatory to conduct energy metabolism, abnormal thyroid function may have adverse effects on blood glucose control in diabetes. The previous study has explored the effect of hypothyroidism on T2DM complication such as nephropathy and cardiovascular events; therefore, diabetic patients need to be screened for thyroid dysfunction.[10]

In this study, primary hypothyroidism was found in 24(40%) diabetic patients(5.67±3.35 μIU/mL) versus 3(10%) controls(1.76±1.19 μIU/mL)(P<0.001). Our results agree with other studies showing a high prevalence of hypothyroidism of 12.5%–32.4% in T2DM.[3],[11]

Among hypothyroid patients, a total of 24, 21(87.5%) showed overt hypothyroidism, while only 3(12.0%) showed subclinical hypothyroidism. In reverse, prior research has shown subclinical hypothyroidism as the most prevalent thyroid dysfunction in DM.[12],[13]

The present study revealed no significant correlation between HbA1c and TSH level or thyroid antibodies in diabetic patients, in accordance with the reports of.[14] However, Billic-Komarica et al.[15] found a significant positive correlation between HbA1c and TSH.

Type1 diabetes has confirmed interconnection with autoimmune thyroid disorders through a common genetic inheritance.[16] It has been reported that the positivity to TPO-Ab antibodies in euthyroid patients with type1 diabetes predicts the progression to eventual hypothyroidism,[17] whereas a few studies were conducted to evaluate thyroid autoimmunity in type2 diabetic patients.[18]

In this study, anti-TPO was found in 66.7%(367.21±234.53) of diabetic patients versus 10%(31.78±32.14) of controls (P > 0.001). Anti-Tg was found in 60%(499.98±358.14) of diabetic patients versus 0(53.27±36.23) controls(P > 0.001). These results are in agreement with other studies.[19],[20] However, other studies showed that the prevalence of antithyroid antibodies in diabetic patients was comparable to nondiabetic controls.[21],[22]

A significant positive correlation was found between both TPO antibodies and Tg antibodies and TSH(P=0.05 and P =0.001, respectively) in diabetic patients. This suggests an autoimmune pathogenetic mechanism for thyroid dysfunction in the patients with T2DM, agreeing with Afkhami-Ardekani et al.[21] and Moslem et al.[23]

A total of 28 diabetic patients(46.6%) were both anti-TPO and anti-Tg positive. This suggests that there is much overlap between both thyroid antibodies. Among diabetic patients, who showed hypothyroidism,[24] 3(12.5%) had negative antibodies, 5(20.8%) had only anti-TPO positive, 2(8.3%) had only anti-Tg positive, and 14(58.33%) had both antibodies positive. Among euthyroid diabetic patients(34), 9(25.0%) had both antibodies negative, 7(19.44%) had only anti-TPO positive, 6(16.66%) had only anti-Tg positive, and 14(38.88%) had both antibodies positive, which points out the importance of following up these patients, as they are expected to develop hypothyroidism in the future.

The exact interpretation for the increased incidence of autoimmune thyroid disease in T2DM is still unclear. It is believed to be due to genetic and environmental factors, infections, and psychological stress. Another possible explanation could be the presence of GAD antibodies in some patients of T2DM.[24] Autoimmune aspects in T2DM could be a possible link. It is believed that insulin resistance arises when B cells and other immune cells react against the self-antigens. Bcells have been found to accumulate in the visceral fat of obese mice. The resulting self-antigens can trigger an autoimmune response, which in turn accelerates β-cell death.

Researchers recently found that anti-CD20, which targets and eliminates mature B cells, IL1-receptor antagonists(Anakinra), and a T-cell costimulation blocker(abatacept), may be beneficial in improving glucose control in T2DM.[20]

Correlation between thyroid dysfunction and the different parameters of metabolic syndrome could not be found in diabetic patients. On the other hand, higher TSH levels have been documented in patients with metabolic syndrome compared to a nonmetabolic syndrome group, indicating that subclinical hypothyroidism may be a risk factor for metabolic syndrome. This suggests that thyroid dysfunction may increase cardiovascular disease risk in diabetic patients through interconnections with dyslipidemia, insulin resistance, and vascular endothelial dysfunction.[25]


  Conclusion Top


Hypothyroidism was found to be more common in Kurdish women with T2DM compared to controls. The significant positive correlation between both antithyroid antibodies and serum TSH in T2DM patients suggests that thyroid dysfunction in women with T2DM is due to an autoimmune-mediated pathogenetic mechanism, thus declaring the role of autoimmunity in the pathogenesis of T2DM.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
CanarisGJ, ManowitzNR, MayorG, RidgwayEC. The colorado thyroid disease prevalence study. Arch Intern Med 2000;160:526-34.  Back to cited text no. 1
    
2.
WildS, RoglicG, GreenA, SicreeR, KingH. Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.  Back to cited text no. 2
    
3.
Ahmed AA, Mohamed SB, Elmadi SA, Abdorabo AA, Ismail IM, Ismail AM. Assessment of thyroid dysfunctions in type 2 diabetes mellitus patients in Surman, Western-Libya. Int J Clin Exper Med Sci 2017;3:1-4.  Back to cited text no. 3
    
4.
FeelyJ, IslesTE. Screening for thyroid dysfunction in diabetics. Br Med J 1979;1:1678.  Back to cited text no. 4
    
5.
PerrosP, McCrimmonRJ, ShawG, FrierBM. Frequency of thyroid dysfunction in diabetic patients: Value of annual screening. Diabet Med 1995;12:622-7.  Back to cited text no. 5
    
6.
SmithsonMJ. Screening for thyroid dysfunction in a community population of diabetic patients. Diabet Med 1998;15:148-50.  Back to cited text no. 6
    
7.
TagamiT, TamanahaT, ShimazuS, HondaK, NanbaK, NomuraH, etal. Lipid profiles in the untreated patients with Hashimoto thyroiditis and the effects of thyroxine treatment on subclinical hypothyroidism with Hashimoto thyroiditis. Endocr J 2010;57:253-8.  Back to cited text no. 7
    
8.
Murray RK, Granner DK, Mayes PA, Rodwell VW. Harper's Biochemistry. 24th ed., Vol. 24. Stamford, CT: Appleton & Lange; 1996. p. 237, 868.  Back to cited text no. 8
    
9.
NederstigtC, CorssmitEP, de KoningEJ, DekkersOM. Incidence and prevalence of thyroid dysfunction in type1 diabetes. JDiabetes Complications 2016;30:420-5.  Back to cited text no. 9
    
10.
ChenHS, WuTE, JapTS, LuRA, WangML, ChenRL, etal. Subclinical hypothyroidism is a risk factor for nephropathy and cardiovascular diseases in type2 diabetic patients. Diabet Med 2007;24:1336-44.  Back to cited text no. 10
    
11.
GhazaliSM, AbbiyesukuFM. Thyroid dysfunction in type2 diabetics seen at the University College Hospital, Ibadan, Nigeria. Niger J Physiol Sci 2010;25:173-9.  Back to cited text no. 11
    
12.
DemitrostL, RanabirS. Thyroid dysfunction in type2 diabetes mellitus: Aretrospective study. Indian J Endocrinol Metab 2012;16:S334-5.  Back to cited text no. 12
    
13.
FularaSN, FularaNY. Toxic multinodular goitre(Thyrotoxicosis with hyperthyroidism) induced cardiomyopathy: Acase report. Open J Clin Diagn 2017;7:6.  Back to cited text no. 13
    
14.
MaskeyR, ShakyaDR, BaranwalJK, LavajuP, KarkiP, PoudelSK, etal. Hypothyroidism in diabetes mellitus patients in Eastern Nepal. Indian J Endocrinol Metab 2015;19:411-5.  Back to cited text no. 14
    
15.
Billic-KomaricaE, BeciragicA, JunuzovicD. The importance of HbA1c control in patients with subclinical hypothyroidism. Mater Sociomed 2012;24:212-9.  Back to cited text no. 15
    
16.
KawasakiE. Type1 diabetes and autoimmunity. Clin Pediatr Endocrinol 2014;23:99-105.  Back to cited text no. 16
    
17.
Fernández-Castañer M, MolinaA, López-Jiménez L, Gómez JM, SolerJ. Clinical presentation and early course of type1 diabetes in patients with and without thyroid autoimmunity. Diabetes Care 1999;22:377-81.  Back to cited text no. 17
    
18.
AkbarDH, AhmedMM, Al-MughalesJ. Thyroid dysfunction and thyroid autoimmunity in saudi type2 diabetics. Acta Diabetol 2006;43:14-8.  Back to cited text no. 18
    
19.
Yasmin T, Ghafoor F, Malik T, S RN, Khan AU. Pattern of thyroid autoimmunity in type 1 and type 2 diabetics. J Coll Physicians Surg Pak 2006;16:751-4.  Back to cited text no. 19
    
20.
ItariuBK, StulnigTM. Autoimmune aspects of type2 diabetes mellitus–A mini-review. Gerontology 2014;60:189-96.  Back to cited text no. 20
    
21.
Afkhami-ArdekaniM, Shojaoddiny-ArdekaniA, RashidiM. Prevalence of thyroid autoantibodies in type2 diabetic patients. Iran J Diabetes Obes 2012;4:1-6.  Back to cited text no. 21
    
22.
ChubbSA, DavisWA, InmanZ, DavisTM. Prevalence and progression of subclinical hypothyroidism in women with type2 diabetes: The Fremantle Diabetes Study. Clin Endocrinol(Oxf) 2005;62:480-6.  Back to cited text no. 22
    
23.
Moslem F, Sarker Bithi T, Biswas A. Prevalence of thyroid dysfunction among type-2 diabetes patients in an urban diabetes hospital, Bangladesh. Open Sci J Clin Med 2015;3:98-102.  Back to cited text no. 23
    
24.
WittingV, BergisD, SadetD, BadenhoopK. Thyroid disease in insulin-treated patients with type2 diabetes: Aretrospective study. Thyroid Res 2014;7:2.  Back to cited text no. 24
    
25.
LaiY, WangJ, JiangF, WangB, ChenY, LiM, etal. The relationship between serum thyrotropin and components of metabolic syndrome. Endocr J 2011;58:23-30.  Back to cited text no. 25
    


    Figures

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