| ORIGINAL ARTICLE |
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| Year : 2018 | Volume
: 15
| Issue : 2 | Page : 145-149 |
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Increased transforming growth factor-β and interleukin-17 transcripts in peripheral blood of breast cancer patients with different clinical stages
Hadeel Saleem Touma, Wafaa Sadoon Shani
Department of Biology, College of Science, University of Basrah, Basrah, Iraq
Correspondence Address:
Hadeel Saleem Touma
Department of Biology, College of Science, University of Basrah, Basrah
Iraq
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/MJBL.MJBL_26_18
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Background: Currently, cancer as major problem around the world threatens human health and has a high incidence in developing countries. Many reports have showed that there is a dysfunction in the immune system of cancer patients. Interleukin-17 (IL-17) and transforming growth factor-β (TGF-β) are increased in the generated through cancers, and IL-17 is considered as a pro-inflammatory cytokine; IL-17 is an angiogenic factor and promotes tumor growth. Materials and Methods: In this study TGF-β and IL-17, mRNA gene expression has been measured in peripheral blood of 35 breast cancer patients and 35 normal age-matched women using quantitative real-time polymerase chain reaction method with master mix reaction containing SYBER Green. β-actin gene was used as housekeeping gene. Results: Our data demonstrated a significant upregulation of TGF-β and IL-17 gene expression (P < 0.05) in patient's peripheral blood compared to normal healthy control. Levels of genes expression were higher in the breast cancer group compared to the control group. Conclusion: TGF-β and IL-17 expression could be used as a diagnostic marker for detecting breast cancer.
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