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Table of Contents
ORIGINAL ARTICLE
Year : 2021  |  Volume : 18  |  Issue : 2  |  Page : 83-88

How reliable are general histopathologists in detection of helicobacter pylori on routine histology?


Department of Pathology, College of Dentistry, Hawler Medical University, Erbil, Iraq; Examinations, RCPath, London, England, UK

Date of Submission04-Dec-2020
Date of Acceptance09-Feb-2021
Date of Web Publication26-Jun-2021

Correspondence Address:
Saman S Abdulla
Department of Pathology, College of Dentistry, Hawler Medical University, Erbil

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/MJBL.MJBL_94_20

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  Abstract 


Background: Many studies agree that routine histology is less sensitive and less specific than special stains and immunohistochemistry (IHC) with false-positive and false-negative rates. Objectives: The aim was to evaluate the accuracy of four general histopathologists in documenting Helicobacter pylori (Hp) bacteria in gastric biopsies (stained with H and E) by using IHC and modified Giemsa stain (MG), also to assess the specificity of MG and whether it can replace IHC. Materials and Methods: Twenty seven reported gastric biopsies were collected from each of four pathologists (9 reported as negative for Hp, 9 reported as scant Hp, and 9 as abundant Hp). The pathologists selected were known to have different thresholds for Hp. The biopsies were stained with MG and IHC and they were read by two pathologists. Results: The four pathologists showed different thresholds for labeling luminal gastric pits' structures as Hp. Overall, there were 5/36 false-negative cases (13.9%) and 26/72 false-positive cases (36%), most of them in the category reported as scant Hp detected. The results of MG were concordant with that of IHC in 107/108 cases. Conclusion: The overall accuracy rate of Hp detection on routine histology was 71%; false positivity was much more common than false negativity, especially when only scant Hp reported to be present. MG is a reliable stain and if done properly, it can replace the more costly IHC. When Hp identification is crucial from the clinical management of view, special stains and/or IHC should be requested.

Keywords: Gastritis, Helicobacter pylori, immunohistochemistry, modified giemsa stain, routine histology


How to cite this article:
Abdulla SS. How reliable are general histopathologists in detection of helicobacter pylori on routine histology?. Med J Babylon 2021;18:83-8

How to cite this URL:
Abdulla SS. How reliable are general histopathologists in detection of helicobacter pylori on routine histology?. Med J Babylon [serial online] 2021 [cited 2021 Nov 29];18:83-8. Available from: https://www.medjbabylon.org/text.asp?2021/18/2/83/319515




  Introduction Top


Since the discovery of Helicobacter pylori (Hp) bacteria in 1984 by Marshal and Warren,[1] its role in the pathogenesis of chronic gastritis, peptic ulcer disease, MALTOMA, and gastric adenocarcinoma was highlighted. Many diagnostic techniques have been developed which include invasive and noninvasive tests. The usefulness, advantages, disadvantages, false negativity, and false positivity of those tests are heavily investigated.[2],[3],[4] Despite the availability of those tests for the detection of Hp bacteria, none of them is universally accepted as the gold standard. In one study published in 2014, nested polymerase chain reaction was regarded to be the gold standard for Hp detection.[5] Logically, the availability of many reliable noninvasive tests should have made invasive tests (gastroscopy and biopsy) obsolete, taking into account that histology is time consuming, labor intensive, and expensive. Nonetheless, upper gastric endoscopy is widely performed; it allows visualization of the mucosa and taking biopsies for histological examination.

The microscopic examination allows for the assessment of important features like the severity and the type gastritis, the presence or absence of intestinal metaplasia, gastric atrophy, and any malignant transformation.[6],[7]

In addition, to those histological features, detection of Hp bacteria is one of the important elements of the gastric pathology report as Hp's role is well known in gastric pathologies and is recognized as the most common cause of gastritis and gastric lymphoma.[8]

Hp bacteria can be visualized by routine histology, it appears as a Gram-negative, spiral-shaped bacterium whose main reservoir is the human stomach and sometimes the duodenum.[9],[10]

However, these small bacteria could be easily confused with luminal debris within the gastric epithelial surface, and this is a potential source of inter-observer variability among histopathologists.[11]

To enhance the detection of Hp organisms, many laboratories perform special stains, such as Giemsa stain (GS), modified GS (MG), Genta stain, Warthin–Starry stain (WS), and many others.

Over the years, researchers have investigated the sensitivity of routine histology in detection of the Hp and many researchers compared the sensitivity and specificity of different special stains and immunohistochemistry (IHC) in the detection of Hp bacteria.[12]

Numerous studies concluded that IHC is the best method for Hp detection on histological sections.[13] In addition, IHC is able to distinguish coccoid forms of the bacteria from other luminal non-Hp organisms, it also helps in the differentiation of luminal debris and cell borders from proper bacteria and from the observers' point of view IHC slide requires a shorter screening time.[14]

The aim of the study was to assess the accuracy of general histopathologists in detecting Hp organisms on routine histology using IHC and MG and assessment of the sensitivity and specificity of MG in Hp detection and whether it can replace IHC?


  Materials and Methods Top


Study design and samples

Hundred and eight tissue blocks of gastric biopsies (formalin-fixed, paraffin-embedded) were collected from the pathology archive of Rizgary Teaching Hospital and two private laboratories (Erbil city Kurdistan region-Iraq). The cases were reported by four different pathologists with perceived different thresholds of identifying HP in gastric biopsy specimens. For each pathologist, 27 gastric biopsy tissue blocks were collected which were the most recent (from February 2020 going backward), 9 reported as negative for Hp, 9 reported as scant/low-density Hp, and 9 biopsies reported as abundant/numerous Hp identified. The only selection criteria were the Hp status in the pathology report, other details of the pathology reports were ignored like the severity or gastritis, intestinal metaplasia, or other features.

MG stains and Hp IHC were prepared and the slides were read by two of the four pathologists assessed in the study, one of them was perceived to have a low threshold for Hp detection and one was perceived to have high threshold for it.

For the IHC, the paraffin blocks were recut and 4-μm thick sections were prepared. The antibody used was ready-to-use polyclonal rabbit antibody from Dako/Agilent and was prepared using the EnVision FLEX detection system as per the manufacturer's protocol which included standard steps of IHC technique.

For the MG (MGG) staining, after cutting, dewaxing, and steps of rehydration, May Grunwald's was applied for 3 min, followed by washing with a weak stream of tap water, then GS was applied for 17 min, followed by washing again with tap water. Then, the slides were incubated for 10 min at 37° followed by mounting and coverslipping.

The data were analyzed using standard statistical analysis programs.

Ethical consideration

The study was conducted in accordance with the ethical principles that have their origin in the Declaration of Helsinki. The study was approved by the Ethics Committee at Hawler Medical University.


  Results Top


As illustrated in the tale [Table 1], cases reported as negative for Hp, the four pathologists achieved an accuracy of 86.3 (31/36 were correct). The accuracies among the individual four pathologists were 100%, 88.9%, 66.7%, and 88.9%, respectively. Pathologist number 1 (low threshold for Hp detection) achieved 100% accuracy in this category. Pathologist number 4 (high threshold for Hp detection) has one false negative, although he stressed in the report that the morphology of gastritis was highly suggestive of Hp gastritis despite the lack of Hp on routine histology.
Table 1: The results of Helicobacter pylori immunohistochemistry on previously reported gastric biopsies by four pathologists

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In the category where Hp was reported as numerous, the overall accuracy was 83.3 (30/36 correct). The accuracy of the four pathologists were 66.7%, 77%, 88.9%, and 100%, respectively, pathologist number 4 (high threshold for Hp detection) achieved 100% accuracy in this category.

In the third category where Hp bacteria were reported as (scant/low density), the overall accuracy was 44.4% and individually was 22.2%, 44.4%, 55.6, and 55.6%. In this category, pathologist 1 has significant false-positive diagnoses close to 80%, but even pathologist 4 who is perceived to have a high threshold for Hp detection has about 45% false-positive cases. In this study, MG was consistent with IHC in 107/108 cases.


  Discussion Top


Despite the presence of many noninvasive tests for the detection of Hp bacteria, histological detection remains an important tool and can guide the clinician in making proper decisions in individual cases.

There are many histological techniques (special stains and IHC) to assist the pathologist in histological detection of Hp.

Many studies have evaluated the specificity and the sensitivity of those techniques and many of them have shown different detection rates between routine H and E and special stains.[15]

Hp organisms appear as small curved structures in the gastric pits, many other structures could mimic Hp and pathologists have different thresholds in interpreting these luminal findings as Hp [Figure 1].
Figure 1: (a and b) (H and E ×400), Examples of luminal debris and cell borders that is easily recognized and unlikely to be reported as Helicobacter pylori. (c and d) (H and E ×400), Luminal debris and dirt which are easily confused with Helicobacter pylori

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[Figure 1] illustrates debris that is easily recognized as junk (a and b) and debris that is easily confused with Hp bacteria (c and d). [Figures 2] shows debris and proper Hp bacteria on MG (a and b) and debris and proper Hp bacteria on IHC (c and d) [Figures 2].
Figure 2: (a-Modified Giemsa stain ×400) nonbacterial debris, (b-modified Giemsa stain ×400) numerous Helicobacter pylori. (c-immunohistochemistry stain ×400) nonbacterial debris, (d-mmunohistochemistry stain ×400) abundant true Helicobacter pylori bacteria

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In this study, the four pathologists involved are all general pathologists and none of them is a specialist gastrointestinal (GI) pathologist, they are numbered 1, 2, 3, and 4. Number one was perceived by the gastroenterologists as having a low threshold for Hp detection (numerous positive cases), while pathologist 4 was perceived to have a high threshold with numerous negative cases.

The current results confirm that pathologists have different thresholds in interpreting small dark luminal structures in the gastric surface epithelium as true Hp bacteria. It also shows the limitation of routine histology in proper Hp detection when the bacteria are scant.

This variability of pathologists in Hp detection was documented in other studies. A Scandinavian study published in 2013 found that the agreement between four pathologists in the detection of Hp on H and E was moderate. In that study, 82 cases were reviewed and the pathologists made a positive diagnosis in 56% to 84% of the specimens (significant heterogeneity, P < 0.01).[16]

Another study published in 1999 has focused on the pathologist's experience in the bacterial detection. It compared the accuracy of Hp detection by expert and nonexpert pathologists. While the level of experience was not strictly defined, the expert pathologist showed high sensitivity (98.8%) and specificity (100%), as compared to the nonexpert pathologist (sensitivity 92.6%; specificity 90.3%), emphasizing an inter-observer variability influenced by the level of experience.[17] The effect of the expertise of the pathologist is also emphasized in other studies.[18],[19],[20]

However, in our study, all pathologists involved have comparable levels of experience, but they had different training backgrounds.

On the other hand, the agreement in Hp detection was very high (reaching 98%) in another study that included four pathologists (all of them were American board certified). In that study, the pathologists' very high concordance rate was based on the examination of slides stained with the triple stain (Sliver, H and E and Alcan blue at pH of 2.5) and not on routine histology; furthermore, the biopsies in that study were all taken with a jumbo forceps yielding larger than average tissue pieces, and the biopsies were processed by a staff specialized in handling GI samples.[21] Hence, the high rate of concordance achieved in that study cannot be compared to our results.

The false-positive and false-negative cases, which one is more common?

Our study highlighted a significant rate of false-positive Hp detection that reached 36% and most of them in the category of (low-density Hp) group, while the rate of false negatives was about 14%.

This rate of false-positive detection in our study – while still high – is comparable to the findings of another Scandinavian study in which the issue of overdiagnosis (false positivity) was documented.

In that study, four observers assessed the presence of Hp in gastric biopsies taken from 40 patients (20 negative and 20 positive cases confirmed by culture). Histological slides were stained with MG, WS, and IHC. Of the 20 negative cases that were seen by four observers (yielding 80 scores), there were 37, 14, and 8 false-positive scores by all observers for MG, WS, and IHC, respectively.

The authors suggested that non-Hp flora may have accounted for the overdiagnosis of Hp in gastric biopsies. Kappa values for the variance between the four observers were 0.28 for MG, 0.57 for WS, and 0.83 for IHC.[22]

In the above study, the disagreement was based on special stains and IHC and if H and E was used alone, the disagreement could have been wider.

False positivity was also a problem in a Turkish study in which 78 antral biopsies were studied, 66 of them were Hp positive by GS and H and E, but only 56 were positive by IHC.[23]

On the other hand, a study conducted in the UK which included 20 cases reviewed by 20 pathologists creating 400 reports has found that the false-positive diagnoses were much less common than false-negatives, but like our study, the results from cases where Hp was numerous were more consistent, with a detection rate of 89% (as opposed to 90% in our study).[24]

This difference between our findings and the above British study is likely to be due to the nature of training and the threshold every pathologist set for himself in calling luminal structures as Hp organisms, another probable factor is the quality of the histological preparations which can affect the interpretation of luminal debris. The quality of H and E staining can be variable from one laboratory to another and each laboratory should assess its H and E stain quality in the detection of Hp.[25]

Are special stains needed? Is modified Giemsa stain reliable?

Many special stains are available for histological identifications of Hp, carbolfuchsin was one of the first used following Hp discovery.[26] Currently, the commonly used special stains for Hp detection are GS, MG, WS, and Genta stains. Less common stains like the modified Diff-Quik stain have also been used.[27] The sensitivities and specificities of these stains are variable in the literature.

One study reported the sensitivity of Hp detection for MG, WS, and IHC to be 90.0 ± 10.0%, 70.0 ± 14.1%, and 83.8 ± 11.1%, respectively, and the specificities were 53.8 ± 19.3%, 82.5 ± 9.6%, and 90.0 ± 0.0%, respectively.[22]

Another study assessed the usefulness of Genta stain against IHC in which 100 positive cases and 100 negative cases by Genta stain were assessed by IHC, the results showed very good sensitivity and specificity of Genta stain with only three false negative and two false positive recorded.[28]

Another study investigated the sensitivities of H and E, GS, and Genta stain, it found them to be comparable (H and E, 92%; GS, 88%; and Genta, 91%), but the H and E specificity (89%) was significantly lower than that of the special stains (98%). Unsurprisingly, the sensitivity for all these three stains was significantly lower at low Hp density than at high density (H and E, 70% vs. 98%; GS, 64% vs. 96%; Genta, 66% vs. 97%).[11]

In another study, the sensitivity of WS was 94.4%,[3] while triple stain (Steiner, H and E, and Alcian blue at pH 2.5) was used by other researchers and was found to be as sensitive as the WS for the detection of Hp and significantly more sensitive than H and E alone,[29]

Gram's stain was used in another study and the sensitivity and specificity for Hp detection were 92% and 100%, respectively.[30]

MG proved to be more reliable than McMullen and silver stain, but from the observer point of view, the silver stain slide was easier to read because the silver coating makes the bacteria look thick larger.[12]

However, the sensitivity of MG stain was not that promising in another study which included 207 cases, 124 of them were positive by IHC (64.6%). Of those 124 cases, only 91 were positive by MG (73.3%) and only 48 cases were positive by H and E.[13] The sensitivity of MG in the above study was significantly lower than many other studies including ours.

In the above quick review, it is clear that the sensitivity and specificity of those special stains are not consistent. The likely explanation is the quality and to a lesser extent the observer's experience.

In the author's practical experience, the quality of staining techniques is variable in different laboratories for individual stains; in some laboratories, GS is no superior to well-prepared H and E slide, while in other laboratories, the stain is very helpful and the same is true for WS, so the quality of the staining techniques is probably the main reason for the differences of sensitivities and specificities of those special stains, and in the author's experience, if WS is prepared well, it is much easier to read than other special stains.

In the current study, MG was as good as IHC with over 99% (107/108) concordance, although detection of Hp bacteria on the IHC was much easier and less time consuming than the MG.

Is immunohistochemistry needed?

Unlike the variability in the sensitivity and specificity of different special stains for Hp detection, IHC is more consistent and the reliability and superiority of IHC over special stains have been confirmed in numerous studies and review articles.[12],[13],[31]

Some authors emphasized the ability of IHC to detect the coccoid forms of the bacteria. The coccoid form could be easily confused with other non-Hp bacteria and in this situation, IHC is far more reliable than special stains.[22],[23],[32],[33] The coocoid form of the bacteria is thought to be a form of bacterial adaptation to an adversarial environment.[34]

Other studies highlighted the need of IHC in patients treated for Hp eradications, they found that IHC was more accurate than classical methods in identifying any residual Hp after eradication therapy and that IHC should, therefore, be used when the aim is achieving eradication.[35] IHC was also more accurate when the rapid urease test (RUT) is negative (in cases of mild gastritis).[36]

Despite all these published works supporting the use of IHC, an American study recommended that IHC for Hp is not necessary because the sensitivity and specificity of H and E stain between four observers were 100% in third observer and 95% by the fourth observer.[33] The finding of the latter study is not in line with the majority of the published articles that addressed this issue. Besides, the introduction of so many histological special stains in itself is strong evidence proving the limitation of the routine histology. The sensitivity and specificity of detection of HP by routine histology are reported to be between 69%–93% and 78%–90%, respectively.[14]

In the current study, a significant number of false positive cases were identified and confirmed by IHC.


  Conclusion Top


We conclude that general histopathologists have different thresholds for detection of Hp on routine histology depending on their experiences and training background and are more likely to make a false-positive Hp detection. The overall accuracy rate of H and E for Hp detection was 71%.

When the histological identification of the bacteria is essential, gastroenterologists should request further confirmatory histological techniques other than routine histology.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984;1:1311-5.  Back to cited text no. 1
    
2.
Syrjänen K. False positive and false negative results in diagnosis of Helicobacter pylori infection can be avoided by a panel of serum biomarkers (GastroPanel®). Mathews J Gastroenterol Hepatol 2017;2:7.  Back to cited text no. 2
    
3.
Cutler AF, Havstad S, Ma CK, Blaser MJ, Perez-Perez GI, Schubert TT. Accuracy of invasive and noninvasive tests to diagnose Helicobacter pylori infection. Gastroenterology 1995;109:136-41.  Back to cited text no. 3
    
4.
Abadi TBA. Diagnosis of Helicobacter pylori using invasive and noninvasive approaches. J Pathog 2018;2018:1-13.  Back to cited text no. 4
    
5.
Patel SK, Pratap CB, Jain AK, Gulati AK, Nath G. Diagnosis of Helicobacter pylori: What should be the gold standard? World J Gastroenterol 2014;20:12847-59.  Back to cited text no. 5
    
6.
Price AB. The Sydney system: Histological division. J Gastroenterol Hepatol 1991;6:209-22.  Back to cited text no. 6
    
7.
Thaker Y, Moon A, Afzali A. Helicobacter pylori: A review of epidemiology, treatment, and management. J Clin Gastroenterol Treat 2016;2:019.  Back to cited text no. 7
    
8.
Wotherspoon AC, Ortiz-Hidalgo C, Falzon MR, Isaacson PG. Helicobacter pylori-associated gastritis and primary B-cell gastric lymphoma. Lancet 1991;338:1175-6.  Back to cited text no. 8
    
9.
Gravina AG, Zagari RM, De Musis C, Romano L, Loguercio C, Romano M. Helicobacter pylori and extragastric diseases: A review. World J Gastroenterol 2018;24:3204-21.  Back to cited text no. 9
    
10.
Young DG. A stain for demonstrating Helicobacter pylori in gastric biopsies. Biotech Histochem 2001;76:31-4.  Back to cited text no. 10
    
11.
Laine L, Lewin DN, Naritoku W, Cohen H. Prospective comparison of H & E, Giemsa, and Genta stains for the diagnosis of Helicobacter pylori. Gastrointest Endosc 1997;45:463-7.  Back to cited text no. 11
    
12.
Rotimi O, Cairns A, Gray S, Moayyedi P, Dixon MF. Histological identification of Helicobacter pylori: Comparison of staining methods. J Clin Pathol 2000;53:756-9.  Back to cited text no. 12
    
13.
Khdhir BR, Banna H, Hughson MD. Histological demonstration of Helicobacter pylori (H. pylori) in patients with gastritis and peptic ulcer disease (PUD) in Sulaimani. Middle East J Internal Med 2012;5:1-10.  Back to cited text no. 13
    
14.
Lee JY, Kim N. Diagnosis of Helicobacter pylori by invasive test: Histology. Ann Transl Med 2015;3:10.  Back to cited text no. 14
    
15.
Alam El-Din HM, Hashem AG, Ragab YM, Hussein IL, Mohamed DB, Mohamed EB. Evaluation of noninvasive versus invasive techniques for the diagnosis of Helicobacter pylori infection. Appl Immunohistochem Mol Morphol 2013;21:326-33.  Back to cited text no. 15
    
16.
Christensen AH, Gjørup T, Hilden J, Fenger C, Henriksen B, Vyberg M, et al. Observer homogeneity in the histologic diagnosis of Helicobacter pylori. Latent class analysis, kappa coefficient, and repeat frequency. Scand J Gastroenterol 1992;27:933-9.  Back to cited text no. 16
    
17.
MacOni G, Vago L, Galletta G, Imbesi V, Sangaletti O, Parente F, et al. Is routine histological evaluation an accurate test for Helicobacter pylori infection? Aliment Pharmacol Ther 1999;13:327-31.  Back to cited text no. 17
    
18.
Mégraud F. Advantages and disadvantages of current diagnostic tests for the detection of Helicobacter pylori. Scand J Gastroenterol Suppl 1996;215:57-62.  Back to cited text no. 18
    
19.
Sabbagh P, Mohammadnia-Afrouzi M, Javanian M, Babazadeh A, Koppolu V, Vasigala VR, et al. Diagnostic methods for Helicobacter pylori infection: Ideals, options, and limitations. Eur J Clin Microbiol Infect Dis 2019;38:55-66.  Back to cited text no. 19
    
20.
Lee HC, Huang TC, Lin CL, Chen KY, Wang CK, Wu DC. Performance of routine Helicobacter pylori invasive tests in patients with dyspepsia. Gastroenterol Res Pract 2013;2013:1-5.  Back to cited text no. 20
    
21.
El-Zimaity HM, Graham DY, Al-Assi MT, Malaty H, Karttunen TJ, Graham DP, et al. Interobserver variation in the histopathological assessment of Helicobacter pylori gastritis. Hum Pathol 1996;27:35-41.  Back to cited text no. 21
    
22.
Jonkers D, Stobberingh E, de Bruine A, Arends JW, Stockbrügger R. Evaluation of immunohistochemistry for the detection of Helicobacter pylori in gastric mucosal biopsies. J Infect 1997;35:149-54.  Back to cited text no. 22
    
23.
Orhan D, Kalel G, Saltik-Temizel IN, Demir H, Bulun A, Karaağaoğlu E, et al. Immunohistochemical detection of Helicobacter pylori infection in gastric biopsies of urea breath test-positive and -negative pediatric patients. Turk J Pediatr 2008;50:34-9.  Back to cited text no. 23
    
24.
Molyneux AJ, Harris MD. Helicobacter pylori in gastric biopsies–should you trust the pathology report? J R Coll Physicians Lond 1993;27:119-20.  Back to cited text no. 24
    
25.
Hartman DJ, Owens SR. Are routine ancillary stains required to diagnose Helicobacter infection in gastric biopsy specimens? An institutional quality assurance review. Am J Clin Pathol 2012;137:255-60.  Back to cited text no. 25
    
26.
Rocha G, Queiroz D, Mendes E, Lage A, Barbosa A. Simple carbolfuchsin staining for showing C pylori and other spiral bacteria in gastric mucosa. J Clin Pathol 1989;42:1004.  Back to cited text no. 26
    
27.
Potvin CA. A modified diff-quik stain for Helicobacter pylori in gastrointestinal biopsies. Lab Med 1994;25:389-91.  Back to cited text no. 27
    
28.
Toulaymat M, Marconi S, Garb J, Otis C, Nash S. Endoscopic biopsy pathology of Helicobacter pylori gastritis. Comparison of bacterial detection by immunohistochemistry and Genta stain. Arch Pathol Lab Med 1999;123:778-81.  Back to cited text no. 28
    
29.
Genta RM, Robason GO, Graham DY. Simultaneous visualization of Helicobacter pylori and gastric morphology: A new stain. Hum Pathol 1994;25:221-6.  Back to cited text no. 29
    
30.
Montgomery EA, Martin DF, Peura DA. Rapid diagnosis of Campylobacter pylori by Gram's stain. Am J Clin Pathol 1988;90:606-9.  Back to cited text no. 30
    
31.
Boldt MS, Pereira RD, Barbosa AJ. Histological identification of H. pylori stained by hematoxylin-eosin and Giemsa: Review for quality control. J Bras Patol Med Lab 2015;51:108-12.  Back to cited text no. 31
    
32.
Chan WY, Hui PK, Leung KM, Chow J, Kwok F, Ng CS. Coccoid forms of Helicobacter pylori in the human stomach. Am J Clin Pathol 1994;102:503-7.  Back to cited text no. 32
    
33.
Wang XI, Zhang S, Abreo F, Thomas J. The role of routine immunohistochemistry for Helicobacter pylori in gastric biopsy. Ann Diagn Pathol 2010;14:256-9.  Back to cited text no. 33
    
34.
Azevedo NF, Almeida C, Cerqueira L, Dias S, Keevil CW, Vieira MJ. Coccoid form of Helicobacter pylori as a morphological manifestation of cell adaptation to the environment. Appl Environ Microbiol 2007;73:3423-7.  Back to cited text no. 34
    
35.
Marzio L, Angelucci D, Grossi L, Diodoro MG, Di Campli E, Cellini L. Anti-Helicobacter pylori specific antibody immunohistochemistry improves the diagnostic accuracy of Helicobacter pylori in biopsy specimen from patients treated with triple therapy. Am J Gastroenterol 1998;93:223-6.  Back to cited text no. 35
    
36.
Tokunaga Y, Shirahase H, Yamamoto E, Gouda Y, Kanaji K, Ohsumi K. Semiquantitative evaluation for diagnosis of Helicobacter pylori infection in relation to histological changes. Am J Gastroenterol 1998;93:26-9.  Back to cited text no. 36
    


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